ERG27/YLR100W Summary Help

Standard Name ERG27
Systematic Name YLR100W
Feature Type ORF, Verified
Description 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs (1, 2, 3, 4 and see Summary Paragraph)
Name Description ERGosterol biosynthesis
Chromosomal Location
ChrXII:341810 to 342853 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ERG27 GO evidence and references
  View Computational GO annotations for ERG27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
43 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • PCA: 24
  • Two-hybrid: 4

Genetic Interactions
  • Negative Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 347
Molecular Weight (Da) 39,724
Isoelectric Point (pI) 8.9
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:341810 to 342853 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1044 341810..342853 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004090
SUMMARY PARAGRAPH for ERG27

ERG27 encodes 3-keto sterol reductase, an enzyme that catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis (3, 4, 1, 2). Cells lacking ERG27 are sterol auxotrophs, and accumulate noncyclic sterol intermediates when grown in the presence of sterols (3).

Last updated: 2000-09-01 Contact SGD

References cited on this page View Complete Literature Guide for ERG27
1) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
2) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3) Gachotte D, et al.  (1999) Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc Natl Acad Sci U S A 96(22):12655-60
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press