ERG26/YGL001C Summary Help

Standard Name ERG26
Systematic Name YGL001C
Feature Type ORF, Verified
Description C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis (1, 2, 3, 4 and see Summary Paragraph)
Name Description ERGosterol biosynthesis
Chromosomal Location
ChrVII:496502 to 495453 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ERG26 GO evidence and references
  View Computational GO annotations for ERG26
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Pathways
Classical genetics
repressible
Large-scale survey
null
reduction of function
repressible
Resources
136 total interaction(s) for 121 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • PCA: 16
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 76
  • Positive Genetic: 25
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 349
Molecular Weight (Da) 38,706
Isoelectric Point (pI) 6.67
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:496502 to 495453 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1050 496502..495453 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002969
SUMMARY PARAGRAPH for ERG26

ERG26 encodes C-3 sterol dehydrogenase, an enzyme that catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis (3, 4, 1, 2). The enzyme removes the 3-alpha-hydrogen, resulting in the decarboxylation of the 3-ketocarboxylic acid sterol intermediate (3). Cells lacking ERG26 are inviable (3). Mutations in ERG1, which encodes squalene monooxygenase, or in HEM1 or HEM3, which encode heme biosynthetic enzymes, can partially suppress the erg26 null phenotype. The erg26 hem1, erg26 hem3, and erg26 erg1 double mutants can grow if supplemented with ergosterol, and accumulate nonesterified carboxylic acid sterols (3).

Last updated: 2000-09-01 Contact SGD

References cited on this page View Complete Literature Guide for ERG26
1) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
2) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3) Gachotte D, et al.  (1998) Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis. Proc Natl Acad Sci U S A 95(23):13794-9
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press