ERG24/YNL280C Summary 
| Standard Name | ERG24 |
|---|---|
| Systematic Name | YNL280C |
| Feature Type | ORF, Verified |
| Description | C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions (1, 2, 3, 4 and see Summary Paragraph) |
| Name Description | ERGosterol biosynthesis |
| Chromosomal Location | |
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| Note: this feature is encoded on the Crick strand. | |
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Gene Ontology Annotations All ERG24 GO evidence and references
| View Computational GO annotations for ERG24 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| High-throughput |
| Pathways |
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Mutant phenotypes All ERG24 Phenotype evidence and references
interactions All ERG24 Interaction evidence and references
| 52 total interaction(s) for 35 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All ERG24 Protein evidence and references
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Last Update | Coordinates: 2005-11-07 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000005224 |
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SUMMARY PARAGRAPH for ERG24
ERG24 encodes C-14 sterol reductase, an enzyme that acts in ergosterol biosynthesis (3, 4, 1, 2). Cells lacking ERG24 accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions, but cannot grow on rich (YPD) medium under aerobic conditions (3, 5, 6, 7). On defined media, or on YPD supplemented with calcium, erg24 mutants can grow aerobically (7, 8). Erg24p is a target of morpholine antifungals, and erg24 mutations confer resistance to these drugs (9, 8). Expression of ERG9, which encodes farnesyl-diphosphate farnesyl transferase, is increased in erg24 mutants (10).
An ERG24 homolog has been cloned in S. pombe (11), and the human lamin B receptor contains a domain similar to sterol reductases, and can complement the erg24 null phenotype (12).
Last updated: 2000-09-01 
References cited on this page View Complete Literature Guide for ERG24
| 1) | Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6 |
| 2) | Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30 |
| 3) | Lorenz RT and Parks LW (1992) Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol 11(9):685-92 |
| 4) | Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press |
| 5) | Marcireau C, et al. (1992) Construction and growth properties of a yeast strain defective in sterol 14-reductase. Curr Genet 22(4):267-72 |
| 6) | Bard M, et al. (1993) Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28(11):963-7 |
| 7) | Crowley JH, et al. (1996) Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae. J Bacteriol 178(10):2991-3 |
| 8) | Crowley JH, et al. (1998) A calcium-dependent ergosterol mutant of Saccharomyces cerevisiae. Curr Genet 34(2):93-9 |
| 9) | Lai MH, et al. (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140(1):41-9 |
| 10) | Kennedy MA, et al. (1999) Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1445(1):110-22 |
| 11) | Smith S (1995) Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe. Gene 155(1):139-40 |
| 12) | Silve S, et al. (1998) Human lamin B receptor exhibits sterol C14-reductase activity in Saccharomyces cerevisiae. Biochim Biophys Acta 1392(2-3):233-44 |
