ERG13/YML126C Summary

Standard Name	ERG13
Systematic Name	YML126C
Alias	HMGS
Feature Type	ORF, Verified
Description	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis (1, 2 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis

Chromosomal Location	ChrXIII:20535 to 19060 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All ERG13 GO evidence and references

	View Computational GO annotations for ERG13
Molecular Function
Manually curated	hydroxymethylglutaryl-CoA synthase activity (IMP)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP)
Cellular Component
High-throughput	nucleus (IDA)

Pathways	mevalonate pathway superpathway of ergosterol biosynthesis

Mutant phenotypes All ERG13 Phenotype evidence and references

Classical genetics
overexpression	resistance to doxorubicin: increased
unspecified	auxotrophy heat sensitivity: increased
Large-scale survey
conditional	heat sensitivity: increased
null	haploinsufficient inviable
overexpression	resistance to sirolimus: increased resistance to doxorubicin: increased vegetative growth: decreased rate
reduction of function	competitive fitness: decreased resistance to benomyl: decreased resistance to sirolimus: decreased resistance to hydroxyurea: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG13 Interaction evidence and references

	59 total interaction(s) for 52 unique genes/features.
Physical Interactions	Affinity Capture-MS: 31 PCA: 3
Genetic Interactions	Negative Genetic: 13 Positive Genetic: 10 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG13 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:20535 to 19060 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1476	20535..19060	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004595

SUMMARY PARAGRAPH for ERG13

ERG13 encodes hydroxymethylglutaryl-CoA synthase, which catalyzes the formation of hydroxymethylglutaryl-CoA (HMG-CoA) from acteyl-CoA and acetoacetyl-CoA (2). HMG-CoA formation is the second step in the biosynthesis of mevalonate, which is required in turn for the biosynthesis of sterols and nonsterol isoprenoids (3); in yeast, ergosterol is the end product of the sterol biosynthetic pathway (2, 1).

Last updated: 2000-08-21

References cited on this page View Complete Literature Guide for ERG13

1)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
2)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
3)	Stryer L (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company