ERG12/YMR208W Summary Help

Standard Name ERG12 1
Systematic Name YMR208W
Alias RAR1
Feature Type ORF, Verified
Description Mevalonate kinase; acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate (2, 3, 4 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrXIII:684467 to 685798 | ORF Map | GBrowse
Gbrowse
Genetic position: 176 cM
Gene Ontology Annotations All ERG12 GO evidence and references
  View Computational GO annotations for ERG12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
53 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Co-purification: 1

Genetic Interactions
  • Negative Genetic: 35
  • Positive Genetic: 7
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 443
Molecular Weight (Da) 48,459
Isoelectric Point (pI) 5.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:684467 to 685798 | ORF Map | GBrowse
SGD ORF map
Genetic position: 176 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1332 684467..685798 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004821
SUMMARY PARAGRAPH for ERG12

ERG12 encodes mevalonate kinase, a cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol in yeast, from mevalonate (5, 3, 4, 2, 6). Temperature-sensitive erg12 mutants are ergosterol auxotrophs, lack mevalonate kinase activity, and show increased mitotic stability of minichromosomes (1, 7). Mevalonate kinases are found in many other species including rat and human; mutations in the human enzyme are associated with mevalonicaciduria (8 (6, 9).

Last updated: 2000-08-22 Contact SGD

References cited on this page View Complete Literature Guide for ERG12
1) Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77
2) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3) Oulmouden A and Karst F  (1991) Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase. Curr Genet 19(1):9-14
4) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Oulmouden A and Karst F  (1990) Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase. Gene 88(2):253-7
6) Stryer L  (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
7) Kearsey SE and Edwards J  (1987) Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1. Mol Gen Genet 210(3):509-17
8) Tanaka RD, et al.  (1990) Molecular cloning of mevalonate kinase and regulation of its mRNA levels in rat liver. Proc Natl Acad Sci U S A 87(8):2872-6
9) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10