ERG12/YMR208W Summary

Standard Name	ERG12 1
Systematic Name	YMR208W
Alias	RAR1
Feature Type	ORF, Verified
Description	Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate (2, 3, 4 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis 1

Chromosomal Location	ChrXIII:684467 to 685798 \| ORF Map \| GBrowse

	Genetic position: 176 cM

Gene Ontology Annotations All ERG12 GO evidence and references

	View Computational GO annotations for ERG12
Molecular Function
Manually curated	mevalonate kinase activity (IDA)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP) farnesyl diphosphate biosynthetic process, mevalonate pathway (IMP) isopentenyl diphosphate biosynthetic process, mevalonate pathway (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	mevalonate pathway superpathway of ergosterol biosynthesis

Mutant phenotypes All ERG12 Phenotype evidence and references

Classical genetics
null	auxotrophy heat sensitivity: increased
Large-scale survey
conditional	heat sensitivity: increased
null	haploinsufficient inviable resistance to mefloquine: decreased
reduction of function	competitive fitness: increased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG12 Interaction evidence and references

	46 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2
Genetic Interactions	Negative Genetic: 32 Positive Genetic: 6 Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG12 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:684467 to 685798 | |

: 176 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1332	684467..685798	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004821

SUMMARY PARAGRAPH for ERG12

ERG12 encodes mevalonate kinase, a cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol in yeast, from mevalonate (5, 3, 4, 2, 6). Temperature-sensitive erg12 mutants are ergosterol auxotrophs, lack mevalonate kinase activity, and show increased mitotic stability of minichromosomes (1, 7). Mevalonate kinases are found in many other species including rat and human; mutations in the human enzyme are associated with mevalonicaciduria (8 (6, 9).

Last updated: 2000-08-22

References cited on this page View Complete Literature Guide for ERG12

1)	Karst F and Lacroute F (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77
2)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3)	Oulmouden A and Karst F (1991) Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase. Curr Genet 19(1):9-14
4)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5)	Oulmouden A and Karst F (1990) Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase. Gene 88(2):253-7
6)	Stryer L (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
7)	Kearsey SE and Edwards J (1987) Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1. Mol Gen Genet 210(3):509-17
8)	Tanaka RD, et al. (1990) Molecular cloning of mevalonate kinase and regulation of its mRNA levels in rat liver. Proc Natl Acad Sci U S A 87(8):2872-6
9)	Foury F (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10