ERG11/YHR007C Summary Help

Standard Name ERG11 1
Systematic Name YHR007C
Alias CYP51
Feature Type ORF, Verified
Description Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p (2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrVIII:121683 to 120091 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 12 cM
Gene Ontology Annotations All ERG11 GO evidence and references
  View Computational GO annotations for ERG11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Pathways
Classical genetics
null
overexpression
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
unspecified
Resources
285 total interaction(s) for 238 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • PCA: 85
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 2
  • Negative Genetic: 89
  • Phenotypic Enhancement: 1
  • Positive Genetic: 12
  • Synthetic Growth Defect: 31
  • Synthetic Lethality: 44
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 530
Molecular Weight (Da) 60,720
Isoelectric Point (pI) 9.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:121683 to 120091 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 12 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1593 121683..120091 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001049
SUMMARY PARAGRAPH for ERG11

ERG11 encodes lanosterol 14-alpha-demethylase, an enzyme in the cytochrome P450 family that catalyzes the C-14 demethylation of lanosterol to form 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol, a step in ergosterol biosynthesis (8, 5, 7, 9, 3).

The erg11 null mutant requires ergosterol and cannot grow aerobically (5). Expression of the wheat CYP51 gene complements the erg11 null phenotype (10). A mutation in ERG3 can suppress the erg11 null phenotype, and combination of an erg11 mutation with a mutation that reduces heme levels (hem2 or hem4) suppresses the sterol auxotrophy of an erg25 disruption; ERG3 and ERG25 encode enzymes that act downstream of Erg11p in ergosterol biosynthesis (11, 12).

Lanosterol 14-alpha-demethylase is the main target of azole antifungal componds such as fluconazole and ketoconazole in fungi including S. cerevisiae and Candida albicans; the drugs act by interacting with the heme iron (6, 4, ). Overexpression of ERG11 causes resistance to azole antifungals (13, 14).

Last updated: 2000-08-31 Contact SGD

References cited on this page View Complete Literature Guide for ERG11
1) Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77
2) Turi TG and Loper JC  (1992) Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14 alpha-demethylase (ERG11). J Biol Chem 267(3):2046-56
3) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
4) Truan G, et al.  (1994) Cloning and characterization of a yeast cytochrome b5-encoding gene which suppresses ketoconazole hypersensitivity in a NADPH-P-450 reductase-deficient strain. Gene 142(1):123-7
5) Kalb VF, et al.  (1987) Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6(6):529-37
6) Yoshida Y and Aoyama Y  (1987) Interaction of azole antifungal agents with cytochrome P-45014DM purified from Saccharomyces cerevisiae microsomes. Biochem Pharmacol 36(2):229-35
7) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
8) Aoyama Y, et al.  (1981) Evidence for the presence of cytochrome P-450 functional in lanosterol 14 alpha-demethylation in microsomes of aerobically grown respiring yeast. Biochim Biophys Acta 665(3):596-601
9) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
10) Cabello-Hurtado F, et al.  (1999) Optimized expression and catalytic properties of a wheat obtusifoliol 14alpha-demethylase (CYP51) expressed in yeast. Complementation of erg11Delta yeast mutants by plant CYP51. Eur J Biochem 262(2):435-46
11) Bard M, et al.  (1993) Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28(11):963-7
12) Gachotte D, et al.  (1997) A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme. Proc Natl Acad Sci U S A 94(21):11173-8
13) Venkateswarlu K, et al.  (1998) NADPH cytochrome P-450 oxidoreductase and susceptibility to ketoconazole. Antimicrob Agents Chemother 42(7):1756-61
14) Kontoyiannis DP, et al.  (1999) Overexpression of Erg11p by the regulatable GAL1 promoter confers fluconazole resistance in Saccharomyces cerevisiae. Antimicrob Agents Chemother 43(11):2798-800