ERG1/YGR175C Summary Help

Standard Name ERG1 1
Systematic Name YGR175C
Feature Type ORF, Verified
Description Squalene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine (1, 2 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrVII:848423 to 846933 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ERG1 GO evidence and references
  View Computational GO annotations for ERG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
55 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Co-fractionation: 1
  • PCA: 10
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 11
  • Phenotypic Enhancement: 1
  • Positive Genetic: 6
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 496
Molecular Weight (Da) 55,125
Isoelectric Point (pI) 6.45
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:848423 to 846933 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1491 848423..846933 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003407

ERG1 encodes squalene monooxygenase (also called squalene epoxidase), an enzyme that oxidizes squalene to form squalene 2,3-epoxide, an intermediate in ergosterol biosynthesis (1, 3, 4, 5). ERG1 is essential under aerobic growth conditions, but erg1 null mutants are viable when grown under anaerobic conditions in the presence of ergosterol (6). Mutations in erg1 also cause resistance to allylamine (1). The activity of Erg1p varies depending on that availability of oxygen and ergosterol, suggesting that squalene monooxygenase may be involved in regulating sterol biosynthesis (7). Erg1p is found in the endoplasmic reticulum (ER) and in lipid particles, but only the ER pool has enzymatic activity (8).

Last updated: 2000-08-29 Contact SGD

References cited on this page View Complete Literature Guide for ERG1
1) Jandrositz A, et al.  (1991) The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization. Gene 107(1):155-60
2) Satoh T, et al.  (1993) Enzymatic properties of squalene epoxidase from Saccharomyces cerevisiae. Biol Pharm Bull 16(4):349-52
3) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
5) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
6) Landl KM, et al.  (1996) ERG1, encoding squalene epoxidase, is located on the right arm of chromosome VII of Saccharomyces cerevisiae. Yeast 12(6):609-13
7) M'Baya B, et al.  (1989) Regulation of squalene synthetase and squalene epoxidase activities in Saccharomyces cerevisiae. Lipids 24(12):1020-3
8) Leber R, et al.  (1998) Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol Biol Cell 9(2):375-86