ERG1/YGR175C Summary

Standard Name	ERG1
Systematic Name	YGR175C
Feature Type	ORF, Verified
Description	Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine (1, 2 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis

Chromosomal Location	ChrVII:848423 to 846933 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All ERG1 GO evidence and references

	View Computational GO annotations for ERG1
Molecular Function
Manually curated	drug binding (IMP) squalene monooxygenase activity (IDA, IMP)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) lipid particle (IDA)

Pathways	ergosterol biosynthesis superpathway of ergosterol biosynthesis

Mutant phenotypes All ERG1 Phenotype evidence and references

Classical genetics
conditional	auxotrophy heat sensitivity: increased
reduction of function	resistance to terbinafine: decreased
Large-scale survey
null	inviable vegetative growth: normal
reduction of function	competitive fitness: increased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG1 Interaction evidence and references

	56 total interaction(s) for 37 unique genes/features.
Physical Interactions	Affinity Capture-MS: 13 Affinity Capture-RNA: 2 Affinity Capture-Western: 10 Co-fractionation: 1 PCA: 10 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 8 Phenotypic Enhancement: 1 Positive Genetic: 4 Synthetic Growth Defect: 2 Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:848423 to 846933 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1491	848423..846933	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003407

SUMMARY PARAGRAPH for ERG1

ERG1 encodes squalene monooxygenase (also called squalene epoxidase), an enzyme that oxidizes squalene to form squalene 2,3-epoxide, an intermediate in ergosterol biosynthesis (1, 3, 4, 5). ERG1 is essential under aerobic growth conditions, but erg1 null mutants are viable when grown under anaerobic conditions in the presence of ergosterol (6). Mutations in erg1 also cause resistance to allylamine (1). The activity of Erg1p varies depending on that availability of oxygen and ergosterol, suggesting that squalene monooxygenase may be involved in regulating sterol biosynthesis (7). Erg1p is found in the endoplasmic reticulum (ER) and in lipid particles, but only the ER pool has enzymatic activity (8).

Last updated: 2000-08-29

References cited on this page View Complete Literature Guide for ERG1

1)	Jandrositz A, et al. (1991) The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization. Gene 107(1):155-60
2)	Satoh T, et al. (1993) Enzymatic properties of squalene epoxidase from Saccharomyces cerevisiae. Biol Pharm Bull 16(4):349-52
3)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4)	Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
5)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
6)	Landl KM, et al. (1996) ERG1, encoding squalene epoxidase, is located on the right arm of chromosome VII of Saccharomyces cerevisiae. Yeast 12(6):609-13
7)	M'Baya B, et al. (1989) Regulation of squalene synthetase and squalene epoxidase activities in Saccharomyces cerevisiae. Lipids 24(12):1020-3
8)	Leber R, et al. (1998) Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol Biol Cell 9(2):375-86