ENA2/YDR039C Summary

Standard Name	ENA2 1
Systematic Name	YDR039C
Feature Type	ORF, Verified
Description	P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux (1, 2 and see Summary Paragraph)
Name Description	Exitus NAtru (Latin, "exit sodium") 1

Chromosomal Location	ChrIV:534582 to 531307 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All ENA2 GO evidence and references

	View Computational GO annotations for ENA2
Molecular Function
Manually curated	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism (IGI, ISS) potassium-transporting ATPase activity (IMP) sodium-exporting ATPase activity, phosphorylative mechanism (IGI, ISS)
Biological Process
Manually curated	potassium ion transport (IMP) sodium ion transport (IGI) transmembrane transport (IMP)
Cellular Component
Manually curated	plasma membrane (IDA)
High-throughput	integral to membrane (ISM) mitochondrion (IDA)

Mutant phenotypes All ENA2 Phenotype evidence and references

Classical genetics
overexpression	resistance to sodium acetate: increased
Large-scale survey
null	filamentous growth: increased
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ENA2 Interaction evidence and references

	21 total interaction(s) for 19 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Co-fractionation: 2 PCA: 5 Protein-RNA: 1
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Synthetic Growth Defect: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ENA2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:534582 to 531307 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3276	534582..531307	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000002446

SUMMARY PARAGRAPH for ENA2

ENA2 is the second member of a tandem array of genes encoding nearly, but not perfectly, identical P-Type ATPases. The reference strain, S288c, contains three genes in this cluster: ENA1, ENA2, and ENA5. Other Saccharomyces strains typically contain 4 or 5 genes (ENA1-ENA5) (3, 4).

ENA1 is the most well characterized member of this cluster and is thought to encode the primary plasma membrane Na+-ATPase exporter in S. cerevisiae. Ena1p plays a critical role in the detoxification of Na+ ions and in maintaining ion homeostasis, making Ena1p a principal component of the cell's ability to survive high salt or alkaline conditions (5). Ena1p and Ena2p both transport Li+ ions, but with differing efficiencies (3), and have been shown to export K+ ions concomitantly with Na+, at some salt concentrations (6).

Ena1p-Ena5p are closely related to Pmr1p (3), the Golgi membrane Ca2+-ATPase. Mutations in the human homolog of Pmr1p, ATP2C1, cause the acantholytic skin condition Hailey-Hailey disease (7).

Last updated: 2006-10-31

References cited on this page View Complete Literature Guide for ENA2

1)	Haro R, et al. (1991) A novel P-type ATPase from yeast involved in sodium transport. FEBS Lett 291(2):189-91
2)	Garciadeblas B, et al. (1993) Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae. Mol Gen Genet 236(2-3):363-8
3)	Wieland J, et al. (1995) The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane. EMBO J 14(16):3870-82
4)	Martinez R, et al. (1991) A PMR2 tandem repeat with a modified C-terminus is located downstream from the KRS1 gene encoding lysyl-tRNA synthetase in Saccharomyces cerevisiae. Mol Gen Genet 227(1):149-54
5)	Platara M, et al. (2006) The Transcriptional Response of the Yeast Na+-ATPase ENA1 Gene to Alkaline Stress Involves Three Main Signaling Pathways. J Biol Chem 281(48):36632-42
6)	Benito B, et al. (2002) Potassium- or sodium-efflux ATPase, a key enzyme in the evolution of fungi. Microbiology 148(Pt 4):933-41
7)	Marie Mauro T (2004) Yeast researchers consider Hailey-Hailey disease. J Invest Dermatol 123(6):xxii-xxiii