EMP65/YER140W Summary Help

Standard Name EMP65 1
Systematic Name YER140W
Feature Type ORF, Verified
Description ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies (1, 2, 3, 4)
Name Description ER Membrane Protein of 65 kDa 1
Chromosomal Location
ChrV:451565 to 453235 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EMP65 GO evidence and references
  View Computational GO annotations for EMP65
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Large-scale survey
null
overexpression
Resources
102 total interaction(s) for 62 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 2

Genetic Interactions
  • Negative Genetic: 20
  • Phenotypic Enhancement: 50
  • Phenotypic Suppression: 25
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 556
Molecular Weight (Da) 64,793
Isoelectric Point (pI) 10.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:451565 to 453235 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1671 451565..453235 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000942
References cited on this page View Complete Literature Guide for EMP65
1) Friederichs JM, et al.  (2012) Genetic Analysis of Mps3 SUN Domain Mutants in Saccharomyces cerevisiae Reveals an Interaction with the SUN-Like Protein Slp1. G3 (Bethesda) 2(12):1703-18
2) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
3) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
4) Jonikas MC, et al.  (2009) Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum. Science 323(5922):1693-7