EHT1/YBR177C Summary Help

Standard Name EHT1 1
Systematic Name YBR177C
Feature Type ORF, Verified
Description Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Ethanol Hexanoyl Transferase 6
Chromosomal Location
ChrII:586162 to 584807 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All EHT1 GO evidence and references
  View Computational GO annotations for EHT1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
70 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 2
  • PCA: 17
  • Protein-peptide: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 25
  • Phenotypic Enhancement: 1
  • Positive Genetic: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 451
Molecular Weight (Da) 51,255
Isoelectric Point (pI) 7.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:586162 to 584807 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1356 586162..584807 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000381
References cited on this page View Complete Literature Guide for EHT1
1) Saerens SM, et al.  (2006) The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity. J Biol Chem 281(7):4446-56
2) Athenstaedt K, et al.  (1999) Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae. J Bacteriol 181(20):6441-8
3) Baxter SM, et al.  (2004) Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast. Mol Cell Proteomics 3(3):209-25
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
6) Mason AB and Dufour JP  (2000) Alcohol acetyltransferases and the significance of ester synthesis in yeast. Yeast 16(14):1287-98