EFT2/YDR385W Summary

EFT2 BASIC INFORMATION

Standard Name	EFT2 1
Systematic Name	YDR385W
Feature Type	ORF, Verified
Description	Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin (1, 2, 3)
Name Description	Elongation Factor Two 1

GO Annotations	All EFT2 GO evidence and references
	View Computational GO annotations for EFT2
Molecular Function
Manually curated	translation elongation factor activity (TAS)
Biological Process
Manually curated	positive regulation of translational elongation (IMP) translational elongation (TAS)
Cellular Component
Manually curated	ribosome (TAS)

Mutant Phenotype	All EFT2 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased
unspecified	resistance to sordarin: increased
Large-scale survey
null	competitive fitness: decreased resistance to hygromycin B: decreased viable
overexpression	vegetative growth: decreased

Interactions	EFT2 All interactions details and references
	84 total interaction(s) for 73 unique genes/features.
Physical Interactions	Affinity Capture-MS: 50 Affinity Capture-Western: 2 PCA: 6 Two-hybrid: 2
Genetic Interactions	Phenotypic Enhancement: 14 Phenotypic Suppression: 7 Synthetic Growth Defect: 1 Synthetic Lethality: 2

Sequence Information

ChrIV:1243223 to 1245751 | |

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2529	1243223..1245751	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002793

EFT2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for EFT2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for EFT2]

1)	Perentesis JP, et al. (1992) Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling. J Biol Chem 267(2):1190-7
2)	Justice MC, et al. (1998) Elongation factor 2 as a novel target for selective inhibition of fungal protein synthesis. J Biol Chem 273(6):3148-51
3)	Dunlop PC and Bodley JW (1983) Biosynthetic labeling of diphthamide in Saccharomyces cerevisiae. J Biol Chem 258(8):4754-8

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