EFR3/YMR212C Summary Help

Standard Name EFR3 1
Systematic Name YMR212C
Feature Type ORF, Verified
Description Protein required for Stt4-containing PI kinase complex localization; required for Stt4-containing phosphoinositide (PI) kinase patch assembly at the plasma membrane; recruited to the plasma membrane via a conserved basic patch near its N-terminus; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene (1, 2, 3)
Name Description PHO Eighty Five Requiring 1
Chromosomal Location
ChrXIII:693043 to 690695 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All EFR3 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
overexpression
Resources
22 total interaction(s) for 15 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 782
Molecular Weight (Da) 89,190
Isoelectric Point (pI) 7.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:693043 to 690695 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2349 693043..690695 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004825
References cited on this page View Complete Literature Guide for EFR3
1) Lenburg ME and O'Shea EK  (2001) Genetic evidence for a morphogenetic function of the Saccharomyces cerevisiae Pho85 cyclin-dependent kinase. Genetics 157(1):39-51
2) Baird D, et al.  (2008) Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3. J Cell Biol 183(6):1061-74
3) Wu X, et al.  (2014) Structural insights into assembly and regulation of the plasma membrane phosphatidylinositol 4-kinase complex. Dev Cell 28(1):19-29