ECM33/YBR078W Summary

ECM33/YBR078W Summary

Standard Name	ECM33 1
Systematic Name	YBR078W
Feature Type	ORF, Verified
Description	GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	ExtraCellular Mutant 1

Chromosomal Location	ChrII:393123 to 394742 \| ORF Map \| GBrowse

Gene Ontology Annotations All ECM33 GO evidence and references

	View Computational GO annotations for ECM33
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	fungal-type cell wall organization (IMP)
Cellular Component
Manually curated	fungal-type cell wall (IDA) plasma membrane (IDA)
High-throughput	mitochondrion (IDA) plasma membrane (IDA)

Mutant phenotypes All ECM33 Phenotype evidence and references

Classical genetics
null	beta-1,6-glucan excretion: increased beta-1,3-glucan excretion: increased heat sensitivity: increased Hsp150p distribution: abnormal Slt2p modification: increased resistance to caffeine: decreased resistance to sodium dodecyl sulfate: decreased resistance to Calcofluor White: decreased toxin resistance: decreased
unspecified	heat sensitivity: increased killer toxin resistance: decreased resistance to hygromycin B: decreased resistance to enzymatic treatment: decreased
Large-scale survey
null	cell size: increased polyphosphate accumulation: abnormal competitive fitness: decreased competitive fitness: increased dessication resistance: decreased haploinsufficient heat sensitivity: increased oxidative stress resistance: decreased resistance to farnesol: decreased resistance to sirolimus: decreased resistance to propan-1-ol: decreased resistance to neothyonidioside: decreased resistance to wortmannin: normal resistance to elesclomol-Cu: decreased resistance to ethanol: decreased resistance to doxorubicin: decreased sporulation efficiency: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ECM33 Interaction evidence and references

	275 total interaction(s) for 208 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 4 Co-fractionation: 2
Genetic Interactions	Dosage Rescue: 5 Negative Genetic: 227 Phenotypic Enhancement: 2 Positive Genetic: 26 Synthetic Growth Defect: 1 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ECM33 Protein evidence and references

Localization	Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:393123 to 394742 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..58	393123..393180	2011-02-03	1997-01-28
Intron	59..388	393181..393510	2011-02-03	1997-01-28
CDS	389..1620	393511..394742	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000282

References cited on this page View Complete Literature Guide for ECM33

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Bidlingmaier S and Snyder M (2002) Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics 1(6):345-56
3)	Toh-e A and Oguchi T (2002) Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst 77(5):309-22
4)	Terashima H, et al. (2003) The localization change of Ybr078w/Ecm33, a yeast GPI-associated protein, from the plasma membrane to the cell wall, affecting the cellular function. FEMS Microbiol Lett 218(1):175-80
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906