ECM32/YER176W Summary

ECM32/YER176W Summary

Standard Name	ECM32 1
Systematic Name	YER176W
Alias	HEL1 , MTT1
Feature Type	ORF, Verified
Description	DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes (2, 3, 4)
Name Description	ExtraCellular Mutant

Chromosomal Location	ChrV:541690 to 545055 \| ORF Map \| GBrowse

Gene Ontology Annotations All ECM32 GO evidence and references

	View Computational GO annotations for ECM32
Molecular Function
Manually curated	DNA helicase activity (IDA) RNA helicase activity (ISS)
Biological Process
Manually curated	regulation of translational termination (IMP)
Cellular Component
Manually curated	polysome (IDA)

Mutant phenotypes All ECM32 Phenotype evidence and references

Classical genetics
unspecified	resistance to hygromycin B: decreased resistance to papulacandin B: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to benzo[a]pyrene: decreased resistance to cycloheximide: decreased starvation resistance: decreased viable
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ECM32 Interaction evidence and references

	13 total interaction(s) for 13 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 8 Phenotypic Suppression: 1 Positive Genetic: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ECM32 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:541690 to 545055 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3366	541690..545055	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000978

References cited on this page View Complete Literature Guide for ECM32

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Bean DW and Matson SW (1997) Identification of the gene encoding scHelI, a DNA helicase from Saccharomyces cerevisiae. Yeast 13(15):1465-75
3)	Biswas EE, et al. (1995) Biochemical and genetic characterization of a replication protein A dependent DNA helicase from the yeast, Saccharomyces cerevisiae. Biochem Biophys Res Commun 206(3):850-6
4)	Czaplinski K, et al. (2000) Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency. RNA 6(5):730-43