ECM22/YLR228C Summary Help

Standard Name ECM22 1
Systematic Name YLR228C
Feature Type ORF, Verified
Description Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication (2, 3, 4, 5)
Name Description ExtraCellular Mutant
Chromosomal Location
ChrXII:602463 to 600019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ECM22 GO evidence and references
  View Computational GO annotations for ECM22
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 6 genes
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
56 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Negative Genetic: 26
  • Positive Genetic: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 9
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 814
Molecular Weight (Da) 89,285
Isoelectric Point (pI) 6.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:602463 to 600019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2445 602463..600019 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004218
References cited on this page View Complete Literature Guide for ECM22
1) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2) Shianna KV, et al.  (2001) Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. J Bacteriol 183(3):830-4
3) Vik A and Rine J  (2001) Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol 21(19):6395-405
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Marie C, et al.  (2008) Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae. Fungal Genet Biol 45(10):1430-8
6) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
7) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87