DOA4/YDR069C Summary 
| Standard Name | DOA4 1 |
|---|---|
| Systematic Name | YDR069C |
| Alias | DOS1 2 , MUT4 3 , NPI2 4 , 5 , SSV7 6 , UBP4 7 |
| Feature Type | ORF, Verified |
| Description | Ubiquitin isopeptidase; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication (2, 8, 9, 10) |
| Name Description | Degradation Of Alpha 1 |
| Chromosomal Location | |
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| Note: this feature is encoded on the Crick strand. | |
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Gene Ontology Annotations All DOA4 GO evidence and references
| View Computational GO annotations for DOA4 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All DOA4 Phenotype evidence and references
interactions All DOA4 Interaction evidence and references
| 150 total interaction(s) for 110 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All DOA4 Protein evidence and references
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000002476 |
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References cited on this page View Complete Literature Guide for DOA4
| 1) | Papa FR and Hochstrasser M (1993) The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene. Nature 366(6453):313-9 |
| 2) | Singer JD, et al. (1996) Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism. Mol Cell Biol 16(4):1356-66 |
| 3) | Grenson M and Acheroy B (1982) Mutations affecting the activity and the regulation of the general amino-acid permease of Saccharomyces cerevisiae. Localisation of the cis-acting dominant pgr regulatory mutation in the structural gene of this permease. Mol Gen Genet 188(2):261-5 |
| 4) | Jauniaux JC, et al. (1987) Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains. Eur J Biochem 164(3):601-6 |
| 5) | Vandenbol M, et al. (1987) Isolation of the NPR1 gene responsible for the reactivation of ammonia-sensitive amino-acid permeases in Saccharomyces cerevisiae. RNA analysis and gene dosage effects. Eur J Biochem 164(3):607-12 |
| 6) | Xiao W, et al. (1994) UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product. Yeast 10(11):1497-502 |
| 7) | van Nocker S, et al. (1996) The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover. Mol Cell Biol 16(11):6020-8 |
| 8) | Swaminathan S, et al. (1999) The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast. Mol Biol Cell 10(8):2583-94 |
| 9) | Amerik AY, et al. (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80 |
| 10) | Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61 |
