DUT1/YBR252W Summary

DUT1 BASIC INFORMATION

Standard Name	DUT1
Systematic Name	YBR252W
Feature Type	ORF, Verified
Description	dUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability (1, 2, 3)
Name Description	DUTp pyrophosphatase 4

GO Annotations	All DUT1 GO evidence and references
	View Computational GO annotations for DUT1
Molecular Function
Manually curated	dUTP diphosphatase activity (IMP)
Biological Process
Manually curated	pyrimidine deoxyribonucleoside triphosphate catabolic process (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	de novo biosynthesis of pyrimidine deoxyribonucleotides

Mutant Phenotype	All DUT1 Phenotype details and references
Classical genetics
null	auxotrophy
reduction of function	cell cycle progression through the G2/M phase transition: arrested colony size: decreased growth in exponential phase: decreased rate mutation frequency: increased
Large-scale survey
null	inviable
repressible	cell cycle progression in S phase: abnormal

Interactions	DUT1 All interactions details and references
	32 total interaction(s) for 29 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 3 Phenotypic Suppression: 14 Synthetic Growth Defect: 5 Synthetic Lethality: 2

Sequence Information

ChrII:722606 to 723049 | |

Last Update

Coordinates: 2004-07-16 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..444	722606..723049	2004-07-16	1997-01-28

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000456

DUT1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for DUT1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DUT1]

1)	Gadsden MH, et al. (1993) dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. EMBO J 12(11):4425-31
2)	Godsen, M.H, et al. (1986) The isolation of dUTPase gene (DUT1) from Saccharomyces cerevisiae. Yeast 2:121
3)	Guillet M, et al. (2006) dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae. Nucleic Acids Res 34(7):2056-66
4)	McIntosh EM, et al. (1986) Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae. Mol Gen Genet 204(3):363-6

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