DUS3/YLR401C Summary Help

Standard Name DUS3 1
Systematic Name YLR401C
Feature Type ORF, Verified
Description Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress (2, 3, 4)
Name Description DihydroUridine Synthase 1, 3
Chromosomal Location
ChrXII:924448 to 922442 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DUS3 GO evidence and references
  View Computational GO annotations for DUS3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
33 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2
  • Co-purification: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 6
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 668
Molecular Weight (Da) 76,595
Isoelectric Point (pI) 7.38
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:924448 to 922442 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-09 | Sequence: 2006-01-09
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2007 924448..922442 2006-01-09 2006-01-09
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004393
References cited on this page View Complete Literature Guide for DUS3
1) Xing F, et al.  (2004) The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs. J Biol Chem 279(17):17850-60
2) Karpichev IV and Small GM  (1998) Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae. Mol Cell Biol 18(11):6560-70
3) Xing F, et al.  (2002) A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA. RNA 8(3):370-81
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76