DUR1,2/YBR208C Summary 
DUR1,2 BASIC INFORMATION
| Standard Name | DUR1,2 1 |
|---|---|
| Systematic Name | YBR208C |
| Alias | DUR80 |
| Feature Type | ORF, Verified |
| Description | Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation (1, 2, 3 and see Summary Paragraph) 
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| Name Description | Degradation of URea 1 |
| GO Annotations | All DUR1,2 GO evidence and references |
|---|---|
| View Computational GO annotations for DUR1,2 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| High-throughput |
| Pathways |
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| Mutant Phenotype | All DUR1,2 Phenotype details and references |
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| Classical genetics | |
| unspecified | |
| Large-scale survey | |
| null |
| Interactions | DUR1,2 All interactions details and references |
|---|---|
| 11 total interaction(s) for 10 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000000412 |
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DUR1,2 RESOURCES
| SGD ORF map | GBrowse |
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for DUR1,2
SUMMARY PARAGRAPH for DUR1,2
About allantoin degradation
The allantoin degradation pathway, which converts allantoin to ammonia and carbon dioxide, allows S. cerevisiae to use allantoin as a sole nitrogen source. Conversion of allantoin to ammonia is carried out by the DAL1, DAL2, and DAL3 gene products, which work sequentially to generate urea (4). Urea is then degraded to ammonia in a two-step process by the DUR1,2 protein, a multifunctional single enzyme originally thought to be encoded by two tightly-linked genes (hence the name DUR1,2) (1). The allantoin catabolic pathway genes are regulated by a general signal that responds to the availability of readily utilizable nitrogen sources, and also by pathway-specific induction by allantoin or the intermediate allophanate. These regulatory effects are mediated by cis-acting DNA elements and the trans-acting factors Gln3p, Gat1p, Dal80p, Dal81p, and Dal82p (5, 6, 7, 8).
Last updated: 2007-10-03
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DUR1,2]
| 1) | Cooper TG, et al. (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80 |
| 2) | van Vuuren HJ, et al. (1991) Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae. J Bacteriol 173(22):7186-95 |
| 3) | Cox KH, et al. (2000) Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p. J Biol Chem 275(23):17611-8 |
| 4) | Yoo HS, et al. (1985) Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol 5(9):2279-88 |
| 5) | Rai R, et al. (1999) Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. J Biol Chem 274(39):28026-34 |
| 6) | Magasanik B and Kaiser CA (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18 |
| 7) | Scott S, et al. (2000) Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. J Biol Chem 275(10):7198-204 |
| 8) | Talibi D, et al. (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7 |
