DUR1,2/YBR208C Summary Help

Standard Name DUR1,2 1
Systematic Name YBR208C
Alias DUR80
Feature Type ORF, Verified
Description Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description Degradation of URea 1
Chromosomal Location
ChrII:642210 to 636703 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 112 cM
Gene Ontology Annotations All DUR1,2 GO evidence and references
  View Computational GO annotations for DUR1,2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 1 genes
Resources
Pathways
Classical genetics
unspecified
Large-scale survey
null
Resources
22 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14

Genetic Interactions
  • Negative Genetic: 6
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,835
Molecular Weight (Da) 201,830
Isoelectric Point (pI) 5.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:642210 to 636703 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 112 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..5508 642210..636703 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000412
SUMMARY PARAGRAPH for DUR1,2

About allantoin degradation

The allantoin degradation pathway, which converts allantoin to ammonia and carbon dioxide, allows S. cerevisiae to use allantoin as a sole nitrogen source. Conversion of allantoin to ammonia is carried out by the DAL1, DAL2, and DAL3 gene products, which work sequentially to generate urea (5). Urea is then degraded to ammonia in a two-step process by the DUR1,2 protein, a multifunctional single enzyme originally thought to be encoded by two tightly-linked genes (hence the name DUR1,2) (1). The allantoin catabolic pathway genes are regulated by a general signal that responds to the availability of readily utilizable nitrogen sources, and also by pathway-specific induction by allantoin or the intermediate allophanate. These regulatory effects are mediated by cis-acting DNA elements and the trans-acting factors Gln3p, Gat1p, Dal80p, Dal81p, and Dal82p (6, 7, 8, 9).

Last updated: 2007-10-03 Contact SGD

References cited on this page View Complete Literature Guide for DUR1,2
1) Cooper TG, et al.  (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80
2) van Vuuren HJ, et al.  (1991) Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae. J Bacteriol 173(22):7186-95
3) Cox KH, et al.  (2000) Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p. J Biol Chem 275(23):17611-8
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Yoo HS, et al.  (1985) Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol 5(9):2279-88
6) Rai R, et al.  (1999) Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. J Biol Chem 274(39):28026-34
7) Magasanik B and Kaiser CA  (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18
8) Scott S, et al.  (2000) Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. J Biol Chem 275(10):7198-204
9) Talibi D, et al.  (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7