DUO1/YGL061C Summary Help

Standard Name DUO1 1
Systematic Name YGL061C
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (2, 3, 4, 5, 6)
Name Description Death Upon Overproduction 1
Chromosomal Location
ChrVII:389711 to 388968 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DUO1 GO evidence and references
  View Computational GO annotations for DUO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
228 total interaction(s) for 162 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-purification: 6
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 33

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 122
  • Positive Genetic: 15
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 247
Molecular Weight (Da) 27,473
Isoelectric Point (pI) 10.76
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:389711 to 388968 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..744 389711..388968 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003029
References cited on this page View Complete Literature Guide for DUO1
1) Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40
2) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
3) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9
6) Miranda JJ, et al.  (2007) Protein arms in the kinetochore-microtubule interface of the yeast DASH complex. Mol Biol Cell 18(7):2503-10