DUN1/YDL101C Summary Help

Standard Name DUN1 1
Systematic Name YDL101C
Feature Type ORF, Verified
Description Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair (2, 3, 4, 5, 6)
Name Description DNA-damage UNinducible 7
Chromosomal Location
ChrIV:281848 to 280307 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DUN1 GO evidence and references
  View Computational GO annotations for DUN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 5 genes
Classical genetics
Large-scale survey
652 total interaction(s) for 406 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 28
  • Protein-peptide: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 14
  • Dosage Lethality: 160
  • Dosage Rescue: 6
  • Negative Genetic: 180
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 8
  • Positive Genetic: 18
  • Synthetic Growth Defect: 133
  • Synthetic Lethality: 23
  • Synthetic Rescue: 13

Expression Summary
Length (a.a.) 513
Molecular Weight (Da) 58,632
Isoelectric Point (pI) 7.88
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:281848 to 280307 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1542 281848..280307 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002259
References cited on this page View Complete Literature Guide for DUN1
1) Elledge, S.  (1993) Personal Communication, Mortimer Map Edition 12
2) Blanchard H, et al.  (2001) Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases. Acta Crystallogr D Biol Crystallogr 57(Pt 3):459-61
3) Zhao X and Rothstein R  (2002) The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1. Proc Natl Acad Sci U S A 99(6):3746-51
4) Hammet A, et al.  (2002) Posttranscriptional regulation of the RAD5 DNA repair gene by the Dun1 kinase and the Pan2-Pan3 poly(A)-nuclease complex contributes to survival of replication blocks. J Biol Chem 277(25):22469-74
5) Bashkirov VI, et al.  (2003) Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Mol Cell Biol 23(4):1441-52
6) Gupta A, et al.  (2013) Telomere length homeostasis responds to changes in intracellular dNTP pools. Genetics 193(4):1095-105
7) Zhou Z and Elledge SJ  (1993) DUN1 encodes a protein kinase that controls the DNA damage response in yeast. Cell 75(6):1119-27