DUG2/YBR281C Summary Help

Standard Name DUG2 1
Systematic Name YBR281C
Feature Type ORF, Verified
Description Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) (1, 2)
Name Description Deficient in Utilization of Glutathione 1
Chromosomal Location
ChrII:767606 to 764970 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DUG2 GO evidence and references
  View Computational GO annotations for DUG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
Large-scale survey
null
overexpression
Resources
26 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Reconstituted Complex: 2
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 5
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 878
Molecular Weight (Da) 98,070
Isoelectric Point (pI) 5.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:767606 to 764970 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2637 767606..764970 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000485
References cited on this page View Complete Literature Guide for DUG2
1) Ganguli D, et al.  (2007) The Alternative Pathway of Glutathione Degradation Is Mediated by a Novel Protein Complex Involving Three New Genes in Saccharomyces cerevisiae. Genetics 175(3):1137-51
2) Kaur H, et al.  (2012) Glutathione degradation by the alternative pathway (DUG pathway) in Saccharomyces cerevisiae is initiated by (Dug2p-Dug3p)2 complex, a novel glutamine amidotransferase (GATase) enzyme acting on glutathione. J Biol Chem 287(12):8920-31