DUF1/YOL087C Summary Help

Standard Name DUF1 1, 2
Systematic Name YOL087C
Feature Type ORF, Verified
Description Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid (2, 3, 4)
Name Description DUB-associated Factor 1 1
Chromosomal Location
ChrXV:158637 to 155287 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DUF1 GO evidence and references
  View Computational GO annotations for DUF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
59 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Reconstituted Complex: 2

Genetic Interactions
  • Negative Genetic: 18
  • Phenotypic Enhancement: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,116
Molecular Weight (Da) 125,381
Isoelectric Point (pI) 6.67
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:158637 to 155287 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3351 158637..155287 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005447
References cited on this page View Complete Literature Guide for DUF1
1) Bernard D, et al.  (2008) personal communication
2) Pashkova N, et al.  (2010) WD40 repeat propellers define a ubiquitin-binding domain that regulates turnover of F box proteins. Mol Cell 40(3):433-43
3) Rieger KJ, et al.  (1999) Chemotyping of yeast mutants using robotics. Yeast 15(10B):973-86
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91