DST1/YGL043W Summary Help

Standard Name DST1 1
Systematic Name YGL043W
Alias PPR2 2
Feature Type ORF, Verified
Description General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress (3, 4, 5, 6, 7)
Name Description DNA Strand Transfer 1
Gene Product Alias P37 8 , S-II 9 , SII 10 , TFIIS 10
Chromosomal Location
ChrVII:417483 to 418412 | ORF Map | GBrowse
Gbrowse
Genetic position: 24 cM
Gene Ontology Annotations All DST1 GO evidence and references
  View Computational GO annotations for DST1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All DST1 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
overexpression
Resources
interactions All DST1 Interaction evidence and references
425 total interaction(s) for 272 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 192
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 7
  • Positive Genetic: 28
  • Synthetic Growth Defect: 67
  • Synthetic Lethality: 52
  • Synthetic Rescue: 17
Resources
Expression Summary
histogram
Resources
Protein Information All DST1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:417483 to 418412 | ORF Map | GBrowse
SGD ORF map
Genetic position: 24 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..930 417483..418412 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003011
References cited on this page View Complete Literature Guide for DST1
1) Clark AB, et al.  (1991) Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha. Mol Cell Biol 11(5):2576-82
2) Hubert JC, et al.  (1983) Complete sequence of a eukaryotic regulatory gene. EMBO J 2(11):2071-3
3) Davie JK and Kane CM  (2000) Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex. Mol Cell Biol 20(16):5960-73
4) Wu J, et al.  (1996) In vitro characterization of mutant yeast RNA polymerase II with reduced binding for elongation factor TFIIS. Proc Natl Acad Sci U S A 93(21):11552-7
5) Olmsted VK, et al.  (1998) Yeast transcript elongation factor (TFIIS), structure and function. I: NMR structural analysis of the minimal transcriptionally active region. J Biol Chem 273(35):22589-94
6) Ubukata T, et al.  (2003) Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II. J Biol Chem 278(10):8580-5
7) Gomez-Herreros F, et al.  (2012) TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress. Nucleic Acids Res 40(14):6508-19
8) Sawadogo M, et al.  (1980) Similar binding site for P37 factor on yeast RNA polymerases A and B. Biochem Biophys Res Commun 96(1):258-64
9) Nakanishi T, et al.  (1992) Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae. J Biol Chem 267(19):13200-4
10) Davies CJ, et al.  (1990) Homologue of TFIIS in yeast. Nature 345(6273):298