DSL1/YNL258C Summary Help

Standard Name DSL1 1
Systematic Name YNL258C
Alias RNS1 2
Feature Type ORF, Verified
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene (2, 3, 4, 5, 6)
Name Description Dependence on SLy1-20 1
Chromosomal Location
ChrXIV:160373 to 158109 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DSL1 GO evidence and references
  View Computational GO annotations for DSL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
108 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 17
  • Positive Genetic: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 10
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 754
Molecular Weight (Da) 88,071
Isoelectric Point (pI) 4.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:160373 to 158109 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2265 160373..158109 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005202
References cited on this page View Complete Literature Guide for DSL1
1) Vanrheenen SM, et al.  (2001) Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic 2(3):212-31
2) Ishikawa T, et al.  (2005) Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene. J Gen Appl Microbiol 51(1):73-82
3) Andag U, et al.  (2001) The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast. J Biol Chem 276(42):39150-60
4) Reilly BA, et al.  (2001) Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell 12(12):3783-96
5) Tripathi A, et al.  (2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16(2):114-23
6) Wainman A, et al.  (2012) The Drosophila RZZ complex - roles in membrane trafficking and cytokinesis. J Cell Sci 125(Pt 17):4014-25