DSK2/YMR276W Summary Help

Standard Name DSK2 1
Systematic Name YMR276W
Feature Type ORF, Verified
Description Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6, 7, 8)
Chromosomal Location
ChrXIII:818827 to 819948 | ORF Map | GBrowse
Gene Ontology Annotations All DSK2 GO evidence and references
  View Computational GO annotations for DSK2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 18 genes
Classical genetics
Large-scale survey
126 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 27
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Far Western: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 10
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 14
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 5
  • Positive Genetic: 5
  • Synthetic Growth Defect: 7
  • Synthetic Rescue: 7

Expression Summary
Length (a.a.) 373
Molecular Weight (Da) 39,345
Isoelectric Point (pI) 4.63
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:818827 to 819948 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1122 818827..819948 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004889
References cited on this page View Complete Literature Guide for DSK2
1) Vallen EA, et al.  (1994) Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Genetics 137(2):407-22
2) Biggins S, et al.  (1996) Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center. J Cell Biol 133(6):1331-46
3) Khalfan W, et al.  (2000) Functional interaction between the PKC1 pathway and CDC31 network of SPB duplication genes. Genetics 155(4):1543-59
4) Kaye FJ, et al.  (2000) A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch. FEBS Lett 467(2-3):348-55
5) Funakoshi M, et al.  (2002) Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A 99(2):745-50
6) Rao H and Sastry A  (2002) Recognition of specific ubiquitin conjugates is important for the proteolytic functions of the ubiquitin-associated domain proteins Dsk2 and Rad23. J Biol Chem 277(14):11691-5
7) Saeki Y, et al.  (2002) Ubiquitin-like proteins and Rpn10 play cooperative roles in ubiquitin-dependent proteolysis. Biochem Biophys Res Commun 293(3):986-92
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76