DRE2/YKR071C Summary Help

Standard Name DRE2 1
Systematic Name YKR071C
Feature Type ORF, Verified
Description Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress (2, 3, 4, 5)
Name Description Derepressed for Ribosomal protein S14 Expression 1
Chromosomal Location
ChrXI:575980 to 574934 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DRE2 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
reduction of function
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
47 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 10
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 348
Molecular Weight (Da) 38,542
Isoelectric Point (pI) 4.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:575980 to 574934 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1047 575980..574934 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001779
References cited on this page View Complete Literature Guide for DRE2
1) Woolford, J  (2001) personal communication
2) Zhang Y, et al.  (2008) Dre2, a conserved eukaryotic fe/s cluster protein, functions in cytosolic fe/s protein biogenesis. Mol Cell Biol 28(18):5569-82
3) Vernis L, et al.  (2009) A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast. PLoS One 4(2):e4376
4) Netz DJ, et al.  (2010) Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis. Nat Chem Biol 6(10):758-65
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76