DPH6/YLR143W Summary Help

Standard Name DPH6 1, 2
Systematic Name YLR143W
Feature Type ORF, Verified
Description Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene (1, 2, 3, 4)
Name Description DiPHthamide biosynthesis 2
Chromosomal Location
ChrXII:427329 to 429386 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DPH6 GO evidence and references
  View Computational GO annotations for DPH6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
20 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 685
Molecular Weight (Da) 77,940
Isoelectric Point (pI) 5.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:427329 to 429386 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2058 427329..429386 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004133
References cited on this page View Complete Literature Guide for DPH6
1) Su X, et al.  (2012) Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A 109(49):19983-7
2) Uthman S, et al.  (2013) The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network. PLoS Genet 9(2):e1003334
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91