DPH2/YKL191W Summary Help

Standard Name DPH2 1
Systematic Name YKL191W
Feature Type ORF, Verified
Description Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p (2, 3, 4)
Name Description DiPHthamide 2
Chromosomal Location
ChrXI:81035 to 82639 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DPH2 GO evidence and references
  View Computational GO annotations for DPH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
unspecified
Resources
45 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 12
  • Positive Genetic: 1
  • Synthetic Growth Defect: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 534
Molecular Weight (Da) 59,775
Isoelectric Point (pI) 4.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:81035 to 82639 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1605 81035..82639 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001674
References cited on this page View Complete Literature Guide for DPH2
1) Chen JY, et al.  (1985) Diphtheria toxin-resistant mutants of Saccharomyces cerevisiae. Mol Cell Biol 5(12):3357-60
2) Mattheakis LC, et al.  (1993) Diphthamide synthesis in Saccharomyces cerevisiae: structure of the DPH2 gene. Gene 132(1):149-54
3) Fichtner L, et al.  (2003) Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification. Mol Microbiol 49(5):1297-307
4) Liu S, et al.  (2004) Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Mol Cell Biol 24(21):9487-97