DPB11/YJL090C Summary 
| Standard Name | DPB11 1 |
|---|---|
| Systematic Name | YJL090C |
| Feature Type | ORF, Verified |
| Description | DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | DNA Polymerase B (II) 1 |
| Chromosomal Location | |
|---|---|
| Note: this feature is encoded on the Crick strand. | |
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Gene Ontology Annotations All DPB11 GO evidence and references
| View Computational GO annotations for DPB11 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated | |
| High-throughput |
Mutant phenotypes All DPB11 Phenotype evidence and references
| Classical genetics | |
|---|---|
| conditional |
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| repressible | |
| Large-scale survey | |
| conditional | |
| null | |
| reduction of function | |
| repressible | |
| Resources |
interactions All DPB11 Interaction evidence and references
| 186 total interaction(s) for 95 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
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Expression Summary
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| Resources |
Protein Information All DPB11 Protein evidence and references
| Localization | |
|---|---|
| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
sequence information
| Note: this feature is encoded on the Crick strand. | |||||||||||||
| This feature is contained within: 70472 | |||||||||||||
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| Retrieve sequences | |||||||||||||
Analyze Sequence
| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
Resources
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000003626 |
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References cited on this page View Complete Literature Guide for DPB11
| 1) | Araki H, et al. (1995) Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A 92(25):11791-5 |
| 2) | Masumoto H, et al. (2000) Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast. Mol Cell Biol 20(8):2809-17 |
| 3) | Wang H and Elledge SJ (1999) DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(7):3824-9 |
| 4) | Kamimura Y, et al. (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9 |
| 5) | Tanaka S, et al. (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445(7125):328-32 |
| 6) | Navadgi-Patil VM and Burgers PM (2008) Yeast DNA Replication Protein Dpb11 Activates the Mec1/ATR Checkpoint Kinase. J Biol Chem 283(51):35853-9 |
| 7) | Mordes DA, et al. (2008) Dpb11 activates the Mec1-Ddc2 complex. Proc Natl Acad Sci U S A 105(48):18730-4 |
| 8) | Zegerman P and Diffley JF (2007) Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Nature 445(7125):281-5 |
| 9) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
