DOT6/YER088C Summary Help

Standard Name DOT6 1
Systematic Name YER088C
Alias PBF2 2
Feature Type ORF, Verified
Description Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Disruptor Of Telomeric silencing 1
Chromosomal Location
ChrV:335188 to 333176 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DOT6 GO evidence and references
  View Computational GO annotations for DOT6
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
High-throughput
Targets 11 genes
Regulators 8 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
73 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 44
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 670
Molecular Weight (Da) 73,048
Isoelectric Point (pI) 10.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:335188 to 333176 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2013 335188..333176 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000890
References cited on this page View Complete Literature Guide for DOT6
1) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
2) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
3) Lorenz MC and Heitman J  (1998) Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains. Genetics 150(4):1443-57
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Shevchenko A, et al.  (2008) Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment. Genome Biol 9(11):R167
6) Voegtle FN, et al.  (2012) Intermembrane space proteome of yeast mitochondria. Mol Cell Proteomics 11(12):1840-52
7) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87