Standard Name	DOT6 1
Systematic Name	YER088C
Alias	PBF2 2
Feature Type	ORF, Verified
Description	Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description	Disruptor Of Telomeric silencing 1

Chromosomal Location	ChrV:335188 to 333176 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for DOT6
Molecular Function
Manually curated	sequence-specific DNA binding (IDA)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	chromatin silencing at rDNA (IGI) chromatin silencing at telomere (IGI) filamentous growth (IGI) regulation of transcription from RNA polymerase II promoter (IGI, IMP) unidimensional cell growth (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA) Rpd3L complex (IDA) Rpd3L-Expanded complex (IDA)

Binding motifs	CATCD 7 [Search intergenic regions for CATCD] CTCATCG 2 [Search intergenic regions for CTCATCG]
Resources	ScerTF | UniProbe | YEASTRACT | YeTFaSCo

Classical genetics
null	pseudohyphal growth: decreased resistance to sirolimus: increased
overexpression	silencing: decreased
Large-scale survey
null	competitive fitness: increased haploinsufficient resistance to hydroxyurea: increased resistance to benzo[a]pyrene: decreased resistance to cycloheximide: increased resistance to fluconazole: increased resistance to nickel(2+): decreased resistance to bleomycin: decreased resistance to tunicamycin: decreased resistance to sirolimus: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
overexpression	silencing: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	70 total interaction(s) for 60 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Affinity Capture-Western: 1 Biochemical Activity: 2 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 43 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 5 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:335188 to 333176 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2013 335188..333176 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000890