DOM34/YNL001W Summary Help

Standard Name DOM34 1
Systematic Name YNL001W
Feature Type ORF, Verified
Description Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Duplication Of Multilocus region 3
Chromosomal Location
ChrXIV:627456 to 628616 | ORF Map | GBrowse
Gene Ontology Annotations All DOM34 GO evidence and references
  View Computational GO annotations for DOM34
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 2 genes
Classical genetics
Large-scale survey
101 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4
  • Co-crystal Structure: 5
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 30
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 3
  • Synthetic Growth Defect: 36
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 386
Molecular Weight (Da) 44,057
Isoelectric Point (pI) 5.49
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:627456 to 628616 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1161 627456..628616 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004946
References cited on this page View Complete Literature Guide for DOM34
1) Lalo D, et al.  (1994) Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae. Yeast 10(4):523-33
2) Ragan MA, et al.  (1996) An archaebacterial homolog of pelota, a meiotic cell division protein in eukaryotes. FEMS Microbiol Lett 144(2-3):151-5
3) Davis L and Engebrecht J  (1998) Yeast dom34 mutants are defective in multiple developmental pathways and exhibit decreased levels of polyribosomes. Genetics 149(1):45-56
4) Carr-Schmid A, et al.  (2002) Novel G-protein complex whose requirement is linked to the translational status of the cell. Mol Cell Biol 22(8):2564-74
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Lee HH, et al.  (2007) Structural and functional insights into Dom34, a key component of no-go mRNA decay. Mol Cell 27(6):938-50
7) Passos DO, et al.  (2009) Analysis of Dom34 and its function in no-go decay. Mol Biol Cell 20(13):3025-32
8) Shoemaker CJ, et al.  (2010) Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330(6002):369-72
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) van den Elzen AM, et al.  (2014) Dom34-Hbs1 mediated dissociation of inactive 80S ribosomes promotes restart of translation after stress. EMBO J 33(3):265-76
11) Guydosh NR and Green R  (2014) Dom34 rescues ribosomes in 3' untranslated regions. Cell 156(5):950-62