DNF3/YMR162C Summary Help

Standard Name DNF3 1
Systematic Name YMR162C
Feature Type ORF, Verified
Description Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase (1, 2, 3 and see Summary Paragraph)
Name Description Drs2 Neo1 Family 1
Chromosomal Location
ChrXIII:583921 to 578951 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DNF3 GO evidence and references
  View Computational GO annotations for DNF3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Large-scale survey
30 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • PCA: 3
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 7
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,656
Molecular Weight (Da) 188,318
Isoelectric Point (pI) 6.74
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:583921 to 578951 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4971 583921..578951 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000004772

S. cerevisiae has five genes encoding type 4 P-type ATPases: NEO1, DRS2, DNF1, DNF2, and DNF3. The "P-type" designation indicates that these integral membrane proteins form a covalent aspartyl-phosphate catalytic intermediate during ATP hydrolysis (1 and references therein). Most P-type ATPases mediate the transport of small cations across biological membranes. However, members of the "type 4" subfamily are aminophospholipid translocases (flippases), rather than cation transporters, and move phospholipids from one side of a membrane bilayer to the other (reviewed in 4). Of the five S. cerevisiae type 4 P-type ATPases, only NEO1 is essential. Although the four other genes appear to have substantial functional overlap (any single DRS2/DNF gene confers cell viability) (1), they are distinct in their localization, specificity, and cofactor association.

Dnf3p localizes to the trans-Golgi network and it is proposed that phospholipid translocation in Golgi vessicles helps create aminophospholipid asymmetry in membranes en route to the cell surface (2 and references therein). Although it may contribute to other Golgi-related processes as well, intracellular protein transport (1) is the only process in which Dnf3p has a demonstrated role. Dnf3p is associated with the non-catalytic subunit Ynr048Wp (5), and is specific for phosphatidylcholine translocation (2).

The P-type ATPase superfamily is evolutionarily conserved, but the type 4 subfamily is found only in eukaryotes. Fourteen type 4 P-type ATPases have been characterized in humans (4 and references therein), including the DRS2 homolog, ATP8A1 (aka ATPase II) (6) and the DNF1/DNF2 homolog ATP8B1 (aka FIC1) (1). Mutations in ATP8B1 result in progressive familial intrahepatic cholestasis (Byler disease), benign recurrent intrahepatic cholestasis (BRIC), and intrahepatic cholestasis of pregnancy (ICP).

Last updated: 2007-03-01 Contact SGD

References cited on this page View Complete Literature Guide for DNF3
1) Hua Z, et al.  (2002) An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell 13(9):3162-77
2) Alder-Baerens N, et al.  (2006) Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Mol Biol Cell 17(4):1632-42
3) Catty P, et al.  (1997) The complete inventory of the yeast Saccharomyces cerevisiae P-type transport ATPases. FEBS Lett 409(3):325-32
4) Paulusma CC and Oude Elferink RP  (2005) The type 4 subfamily of P-type ATPases, putative aminophospholipid translocases with a role in human disease. Biochim Biophys Acta 1741(1-2):11-24
5) Noji T, et al.  (2006) Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Biochem Biophys Res Commun 344(1):323-31
6) Natarajan P, et al.  (2004) Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function. Proc Natl Acad Sci U S A 101(29):10614-9