DNA2/YHR164C Summary Help

DNA2 BASIC INFORMATION

Standard Name DNA2 1
Systematic Name YHR164C
Alias WEB2
Feature Type ORF, Verified
Description Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization (2, 3, 4, 5, 6, 7)
Name Description DNA synthesis defective 1
GO Annotations All DNA2 GO evidence and references
    View Computational GO annotations for DNA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All DNA2 Phenotype details and references
Classical genetics
conditional
reduction of function
unspecified
Large-scale survey
null
Interactions DNA2 All interactions details and references
102 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-localization: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Rescue: 8
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 37
  • Synthetic Rescue: 8
Sequence Information
ChrVIII:429180 to 424612 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4569 429180..424612 2005-11-07 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001207

DNA2 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for DNA2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DNA2]

1) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
2) Budd ME and Campbell JL  (2000) The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res 459(3):173-86
3) Budd ME and Campbell JL  (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol 17(4):2136-42
4) Schlesinger MB and Formosa T  (2000) POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics 155(4):1593-606
5) Bae SH, et al.  (1998) Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem 273(41):26880-90
6) Bae SH and Seo YS  (2000) Characterization of the enzymatic properties of the yeast dna2 Helicase/endonuclease suggests a new model for Okazaki fragment processing. J Biol Chem 275(48):38022-31
7) Kosugi S, et al.  (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6