DMA1/YHR115C Summary Help

Standard Name DMA1 1
Systematic Name YHR115C
Alias CHF1 2
Feature Type ORF, Verified
Description Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Defective in Mitotic Arrest 1
Chromosomal Location
ChrVIII:341359 to 340109 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DMA1 GO evidence and references
  View Computational GO annotations for DMA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 6 genes
Classical genetics
Large-scale survey
95 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 6
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 14
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 4
  • Positive Genetic: 3
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 16
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 416
Molecular Weight (Da) 46,097
Isoelectric Point (pI) 6.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVIII:341359 to 340109 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1251 341359..340109 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001157
References cited on this page View Complete Literature Guide for DMA1
1) Fraschini R, et al.  (2004) Functional characterization of Dma1 and Dma2, the budding yeast homologues of Schizosaccharomyces pombe Dma1 and human Chfr. Mol Biol Cell 15(8):3796-810
2) Bieganowski P, et al.  (2004) Cdc123 and checkpoint forkhead associated with RING proteins control the cell cycle by controlling eIF2gamma abundance. J Biol Chem 279(43):44656-66
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Tuttle RL, et al.  (2007) Defective in mitotic arrest 1/ring finger 8 is a checkpoint protein that antagonizes the human mitotic exit network. Mol Cancer Res 5(12):1304-11
5) Loring GL, et al.  (2008) Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination. Cell Cycle 7(1):96-105
6) Raspelli E, et al.  (2011) Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization. Mol Biol Cell 22(13):2185-97
7) Merlini L, et al.  (2012) Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the elm1 kinase to the bud neck. PLoS Genet 8(4):e1002670
8) Hernandez-Ortega S, et al.  (2013) Defective in mitotic arrest 1 (dma1) ubiquitin ligase controls g1 cyclin degradation. J Biol Chem 288(7):4704-14