DIG2/YDR480W Summary Help

Standard Name DIG2 1
Systematic Name YDR480W
Alias RST2 2
Feature Type ORF, Verified
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7)
Name Description Down-regulator of Invasive Growth 1
Chromosomal Location
ChrIV:1417399 to 1418370 | ORF Map | GBrowse
Gene Ontology Annotations All DIG2 GO evidence and references
  View Computational GO annotations for DIG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
74 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 7
  • Reconstituted Complex: 6
  • Two-hybrid: 31

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 323
Molecular Weight (Da) 36,879
Isoelectric Point (pI) 7.39
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1417399 to 1418370 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..972 1417399..1418370 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002888
References cited on this page View Complete Literature Guide for DIG2
1) Cook JG, et al.  (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48
2) Tedford K, et al.  (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38
3) Olson KA, et al.  (2000) Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol 20(12):4199-209
4) Bardwell L, et al.  (1998) Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A 95(26):15400-5
5) Gelli A  (2002) Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast. Mol Microbiol 46(3):845-54
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) McCullagh E, et al.  (2010) Coordinate control of gene expression noise and interchromosomal interactions in a MAP kinase pathway. Nat Cell Biol 12(10):954-962