DIG1/YPL049C Summary

DIG1/YPL049C Summary

Standard Name	DIG1 1
Systematic Name	YPL049C
Alias	RST1 2
Feature Type	ORF, Verified
Description	MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7)
Name Description	Down-regulator of Invasive Growth 1

Chromosomal Location	ChrXVI:463839 to 462481 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All DIG1 GO evidence and references

	View Computational GO annotations for DIG1
Molecular Function
Manually curated	transcription factor binding (IDA)
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	negative regulation of invasive growth in response to glucose limitation (IGI) negative regulation of pseudohyphal growth (IGI) negative regulation of transcription from RNA polymerase II promoter by pheromones (IGI)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role

Binding motifs	Predicted DIG1 Binding Site Locations
	TGAAACA 8 [Search intergenic regions for TGAAACA]

Resources	ScerTF \| YeTFaSCo

Mutant phenotypes All DIG1 Phenotype evidence and references

Classical genetics
null	cell fusion: decreased filamentous growth: increased growth in exponential phase: decreased rate invasive growth: increased Fus3p distribution: abnormal Ste12p distribution: abnormal resistance to sirolimus: increased
overexpression	invasive growth: decreased pheromone-induced cell cycle arrest: decreased
Large-scale survey
null	cell cycle progression in G1 phase: delayed competitive fitness: decreased endocytosis: decreased haploinsufficient redox state: abnormal resistance to neomycin: decreased resistance to paromomycin: decreased resistance to tirapazamine: increased resistance to cycloheximide: decreased sporulation efficiency: increased telomere length: increased transposable element transposition: increased vegetative growth: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All DIG1 Interaction evidence and references

	126 total interaction(s) for 77 unique genes/features.
Physical Interactions	Affinity Capture-MS: 26 Affinity Capture-RNA: 2 Affinity Capture-Western: 8 Biochemical Activity: 4 FRET: 1 Protein-RNA: 1 Reconstituted Complex: 6 Two-hybrid: 43
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 6 Phenotypic Enhancement: 6 Phenotypic Suppression: 10 Positive Genetic: 4 Synthetic Growth Defect: 6 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All DIG1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:463839 to 462481 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1359	463839..462481	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005970

References cited on this page View Complete Literature Guide for DIG1

1)	Cook JG, et al. (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48
2)	Tedford K, et al. (1997) Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol 7(4):228-38
3)	Olson KA, et al. (2000) Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol 20(12):4199-209
4)	Bardwell L, et al. (1998) Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A 95(26):15400-5
5)	Gelli A (2002) Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast. Mol Microbiol 46(3):845-54
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7)	McCullagh E, et al. (2010) Coordinate control of gene expression noise and interchromosomal interactions in a MAP kinase pathway. Nat Cell Biol 12(10):954-962
8)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104