DIG1/YPL049C Summary

DIG1 BASIC INFORMATION

Standard Name	DIG1
Systematic Name	YPL049C
Alias	RST1
Feature Type	ORF, Verified
Description	Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription (1, 2, 3, 4)
Name Description	Down-regulator of Invasive Growth 1

GO Annotations	All DIG1 GO evidence and references
	View Computational GO annotations for DIG1
Molecular Function
Manually curated	transcription factor binding (IDA)
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	invasive growth in response to glucose limitation (IGI)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role
Binding motifs	DIG1 Transcription Factor Binding Sites and References
	TGAAACA

Mutant Phenotype	All DIG1 Phenotype details and references
Classical genetics
null	Fus3p distribution: abnormal resistance to rapamycin: increased
Large-scale survey
null	competitive fitness: decreased telomere length: increased transposable element transposition: increased viable

Interactions	DIG1 All interactions details and references
	89 total interaction(s) for 53 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 2 Affinity Capture-Western: 8 Biochemical Activity: 4 FRET: 1 Protein-RNA: 1 Reconstituted Complex: 6 Two-hybrid: 43
Genetic Interactions	Dosage Lethality: 1 Phenotypic Enhancement: 4 Synthetic Growth Defect: 6 Synthetic Lethality: 1 Synthetic Rescue: 1

Sequence Information

ChrXVI:463836 to 462478 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-21 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1359	463836..462478	2004-07-21	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005970

DIG1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for DIG1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DIG1]

1)	Cook JG, et al. (1996) Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev 10(22):2831-48
2)	Olson KA, et al. (2000) Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol 20(12):4199-209
3)	Bardwell L, et al. (1998) Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A 95(26):15400-5
4)	Gelli A (2002) Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast. Mol Microbiol 46(3):845-54

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