Standard Name	DIA2 1
Systematic Name	YOR080W
Alias	YOR29-31
Feature Type	ORF, Verified
Description	Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase (1, 2, 3, 4)
Name Description	Digs Into Agar 1

Chromosomal Location	ChrXV:474594 to 476792 | ORF Map | GBrowse

	View Computational GO annotations for DIA2
Molecular Function
Manually curated	DNA replication origin binding (IPI) contributes_to ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	invasive growth in response to glucose limitation (IGI) protein ubiquitination (IMP) regulation of DNA replication (IMP) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA, IMP)
Cellular Component
Manually curated	colocalizes_with nuclear replication fork (IDA) nucleus (IDA) SCF ubiquitin ligase complex (IDA)

Classical genetics
null	cell cycle progression through the G1/S phase transition: premature chromosome/plasmid maintenance: abnormal colony sectoring: increased prion formation: increased Mrc1p accumulation: increased Rad52p-YFP distribution: abnormal resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to ferrozine(2-): decreased vegetative growth: decreased
overexpression	resistance to methylmercury chloride: normal vegetative growth: decreased
Large-scale survey
null	alkaline pH resistance: decreased anaerobic growth: decreased bud morphology: abnormal cell size: increased competitive fitness: decreased dessication resistance: decreased freeze-thaw resistance: decreased heat sensitivity: increased metal resistance: decreased Fus-Mid-GFP distribution: abnormal resistance to oleate: decreased resistance to CTBT: decreased resistance to sirolimus: decreased resistance to sodium arsenite: decreased resistance to methyl methanesulfonate: decreased resistance to pentachlorophenol: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to doxorubicin: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	560 total interaction(s) for 322 unique genes/features.
Physical Interactions	Affinity Capture-MS: 36 Affinity Capture-Western: 15 Biochemical Activity: 3 Reconstituted Complex: 3 Two-hybrid: 7
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Negative Genetic: 174 Phenotypic Enhancement: 4 Phenotypic Suppression: 6 Positive Genetic: 9 Synthetic Growth Defect: 186 Synthetic Lethality: 113 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:474594 to 476792 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2010-01-05
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2199 474594..476792 2011-02-03 2010-01-05
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005606