DIA2/YOR080W Summary Help

Standard Name DIA2 1
Systematic Name YOR080W
Alias YOR29-31
Feature Type ORF, Verified
Description Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitylation of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth (1, 2, 3, 4, 5, 6)
Name Description Digs Into Agar 1
Chromosomal Location
ChrXV:474594 to 476792 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DIA2 GO evidence and references
  View Computational GO annotations for DIA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
unspecified
Resources
563 total interaction(s) for 323 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 175
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 6
  • Positive Genetic: 9
  • Synthetic Growth Defect: 187
  • Synthetic Lethality: 113
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 732
Molecular Weight (Da) 85,070
Isoelectric Point (pI) 9.29
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:474594 to 476792 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2010-01-05
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2199 474594..476792 2011-02-03 2010-01-05
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005606
References cited on this page View Complete Literature Guide for DIA2
1) Palecek SP, et al.  (2000) Genetic analysis reveals that FLO11 upregulation and cell polarization independently regulate invasive growth in Saccharomyces cerevisiae. Genetics 156(3):1005-23
2) Koepp DM, et al.  (2006) The F-box protein Dia2 regulates DNA replication. Mol Biol Cell 17(4):1540-8
3) Andress EJ, et al.  (2011) Dia2 Controls Transcription by Mediating Assembly of the RSC Complex. PLoS One 6(6):e21172
4) Burgess RJ, et al.  (2012) The SCF(Dia2) Ubiquitin E3 Ligase Ubiquitylates Sir4 and Functions in Transcriptional Silencing. PLoS Genet 8(7):e1002846
5) Kim DH, et al.  (2012) The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. J Biol Chem 287(53):44212-20
6) Fong CM, et al.  (2013) The Saccharomyces cerevisiae F-Box Protein Dia2 Is a Mediator of S-Phase Checkpoint Recovery from DNA Damage. Genetics 193(2):483-99