Standard Name	DEG1
Systematic Name	YFL001W
Alias	PUS3 , HRM3 1
Feature Type	ORF, Verified
Description	tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability (2, 3, 4)
Name Description	DEpressed Growth rate 2

Chromosomal Location	ChrVI:147131 to 148459 | ORF Map | GBrowse
	Genetic position: -1 cM

	View Computational GO annotations for DEG1
Molecular Function
Manually curated	pseudouridine synthase activity (IDA, IMP)
Biological Process
Manually curated	tRNA pseudouridine synthesis (IDA, IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	oxidative stress resistance: decreased resistance to tellurite: decreased
unspecified	replicative lifespan: decreased
Large-scale survey
null	bud morphology: abnormal cell size: increased proton accumulation: increased competitive fitness: decreased freeze-thaw resistance: decreased haploproficient heat sensitivity: increased hyperosmotic stress resistance: decreased oxidative stress resistance: decreased resistance to camptothecin: increased resistance to cycloheximide: increased resistance to fenpropimorph: increased resistance to fluconazole: increased resistance to tunicamycin: increased resistance to methyl methanesulfonate: decreased resistance to nickel sulfate: decreased resistance to arsenite(3-): decreased resistance to caffeine: decreased resistance to sirolimus: decreased resistance to sodium arsenite: decreased resistance to quinine: decreased resistance to dimethyl sulfoxide: decreased resistance to bleomycin: increased resistance to benzo[a]pyrene: decreased stress resistance: increased transposable element transposition: decreased vegetative growth: decreased rate
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	436 total interaction(s) for 301 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Biochemical Activity: 6 Reconstituted Complex: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 298 Phenotypic Enhancement: 5 Phenotypic Suppression: 4 Positive Genetic: 104 Synthetic Growth Defect: 12 Synthetic Lethality: 3 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVI:147131 to 148459 | ORF Map | GBrowse
	Genetic position: -1 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1329 147131..148459 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001895