DED1/YOR204W Summary Help

Standard Name DED1
Systematic Name YOR204W
Alias SPP81
Feature Type ORF, Verified
Description ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase; required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility; DED1 has a paralog, DBP1, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description DEaD-box protein
Chromosomal Location
ChrXV:722911 to 724725 | ORF Map | GBrowse
Gbrowse
Genetic position: 114 cM
Gene Ontology Annotations All DED1 GO evidence and references
  View Computational GO annotations for DED1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
Resources
267 total interaction(s) for 215 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 184
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-purification: 1
  • PCA: 11
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 8
  • Negative Genetic: 16
  • Phenotypic Suppression: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 10
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 604
Molecular Weight (Da) 65,552
Isoelectric Point (pI) 7.88
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:722911 to 724725 | ORF Map | GBrowse
SGD ORF map
Genetic position: 114 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1815 722911..724725 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005730
References cited on this page View Complete Literature Guide for DED1
1) Iost I, et al.  (1999) Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase. J Biol Chem 274(25):17677-83
2) Noueiry AO, et al.  (2000) A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA. Proc Natl Acad Sci U S A 97(24):12985-90
3) Johnstone O, et al.  (2005) Belle is a Drosophila DEAD-box protein required for viability and in the germ line. Dev Biol 277(1):92-101
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61