DDC1/YPL194W Summary Help

Standard Name DDC1 1
Systematic Name YPL194W
Feature Type ORF, Verified
Description DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress (2, 3, 4, 5)
Name Description DNA Damage Checkpoint 1
Chromosomal Location
ChrXVI:179277 to 181115 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DDC1 GO evidence and references
  View Computational GO annotations for DDC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
unspecified
Resources
298 total interaction(s) for 148 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 3
  • Co-fractionation: 2
  • Co-localization: 3
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 125
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 4
  • Positive Genetic: 12
  • Synthetic Growth Defect: 63
  • Synthetic Lethality: 26
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 612
Molecular Weight (Da) 69,693
Isoelectric Point (pI) 6.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:179277 to 181115 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1839 179277..181115 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006115
References cited on this page View Complete Literature Guide for DDC1
1) Longhese MP, et al.  (1997) The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast. EMBO J 16(17):5216-26
2) Hong EJ and Roeder GS  (2002) A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev 16(3):363-76
3) Wang H and Elledge SJ  (2002) Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway. Genetics 160(4):1295-304
4) Giannattasio M, et al.  (2003) Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1. J Biol Chem 278(25):22303-8
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76