DBP2/YNL112W Summary Help

Standard Name DBP2 1
Systematic Name YNL112W
Feature Type ORF, Verified
Description ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; interacts with YRA1; required for the assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites (2, 3, 4)
Name Description Dead Box Protein 1
Chromosomal Location
ChrXIV:413639 to 416281 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DBP2 GO evidence and references
  View Computational GO annotations for DBP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
reduction of function
Resources
106 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 64
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 17
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 546
Molecular Weight (Da) 60,999
Isoelectric Point (pI) 9.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:413639 to 416281 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1273 413639..414911 2011-02-03 1996-07-31
Intron 1274..2275 414912..415913 2011-02-03 1996-07-31
CDS 2276..2643 415914..416281 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005056
References cited on this page View Complete Literature Guide for DBP2
1) Barta I and Iggo R  (1995) Autoregulation of expression of the yeast Dbp2p 'DEAD-box' protein is mediated by sequences in the conserved DBP2 intron. EMBO J 14(15):3800-8
2) Bond AT, et al.  (2001) Absence of Dbp2p alters both nonsense-mediated mRNA decay and rRNA processing. Mol Cell Biol 21(21):7366-79
3) Cloutier SC, et al.  (2012) The DEAD-box RNA helicase Dbp2 connects RNA quality control with repression of aberrant transcription. J Biol Chem 287(31):26155-66
4) Ma WK, et al.  (2013) The DEAD-box Protein Dbp2 Functions with the RNA-Binding Protein Yra1 to Promote mRNP Assembly. J Mol Biol 425(20):3824-3838