DAL3/YIR032C Summary Help

Standard Name DAL3 1
Systematic Name YIR032C
Feature Type ORF, Verified
Description Ureidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to "ureidoglycolate hydrolase" but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Degradation of Allantoin
Chromosomal Location
ChrIX:415617 to 415030 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 37 cM
Gene Ontology Annotations All DAL3 GO evidence and references
  View Computational GO annotations for DAL3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
19 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Protein-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 5
  • Positive Genetic: 6
  • Synthetic Growth Defect: 1

Expression Summary
Length (a.a.) 195
Molecular Weight (Da) 21,727
Isoelectric Point (pI) 5.17
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:415617 to 415030 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 37 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..588 415617..415030 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001471

About allantoin degradation

The allantoin degradation pathway, which converts allantoin to ammonia and carbon dioxide, allows S. cerevisiae to use allantoin as a sole nitrogen source. Conversion of allantoin to ammonia is carried out by the DAL1, DAL2, and DAL3 gene products, which work sequentially to generate urea (1). Urea is then degraded to ammonia in a two-step process by the DUR1,2 protein, a multifunctional single enzyme originally thought to be encoded by two tightly-linked genes (hence the name DUR1,2) (6). The allantoin catabolic pathway genes are regulated by a general signal that responds to the availability of readily utilizable nitrogen sources, and also by pathway-specific induction by allantoin or the intermediate allophanate. These regulatory effects are mediated by cis-acting DNA elements and the trans-acting factors Gln3p, Gat1p, Dal80p, Dal81p, and Dal82p (7, 8, 9, 10).

Last updated: 2007-10-03 Contact SGD

References cited on this page View Complete Literature Guide for DAL3
1) Yoo HS, et al.  (1985) Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol 5(9):2279-88
2) Yoo HS and Cooper TG  (1991) The ureidoglycollate hydrolase (DAL3) gene in Saccharomyces cerevisiae. Yeast 7(7):693-8
3) Magasanik B  (1992) "Regulation of Nitrogen Utilization." Pp. 283-317 in the Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Percudani R, et al.  (2013) Ureidoglycolate hydrolase, amidohydrolase, lyase: how errors in biological databases are incorporated in scientific papers and vice versa. Database (Oxford) 2013(0):bat071
5) Choi KS, et al.  (1966) The assay of yeast ureidoglycolatase. Anal Biochem 17(3):413-22
6) Cooper TG, et al.  (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80
7) Rai R, et al.  (1999) Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. J Biol Chem 274(39):28026-34
8) Magasanik B and Kaiser CA  (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18
9) Scott S, et al.  (2000) Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. J Biol Chem 275(10):7198-204
10) Talibi D, et al.  (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7