DAD4/YDR320C-A Summary Help

Standard Name DAD4 1
Systematic Name YDR320C-A
Alias HSK2 2
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (1, 3, 4, 5, 6)
Name Description Duo1 And Dam1 interacting 1
Chromosomal Location
ChrIV:1108498 to 1108280 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DAD4 GO evidence and references
  View Computational GO annotations for DAD4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
57 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-crystal Structure: 1
  • Co-purification: 6
  • PCA: 3
  • Two-hybrid: 34

Genetic Interactions
  • Dosage Rescue: 1

Expression Summary
Length (a.a.) 72
Molecular Weight (Da) 8,155
Isoelectric Point (pI) 7.46
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1108498 to 1108280 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2001-02-26
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..219 1108498..1108280 2011-02-03 2001-02-26
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007604
References cited on this page View Complete Literature Guide for DAD4
1) Cheeseman IM, et al.  (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2):163-72
2) Li JM, et al.  (2005) Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association. Mol Cell Biol 25(2):767-78
3) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
4) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
5) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
6) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9