DAD1/YDR016C Summary Help

Standard Name DAD1 1
Systematic Name YDR016C
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (1, 2, 3, 4)
Name Description Duo1 And Dam1 interacting 1
Chromosomal Location
ChrIV:478758 to 478474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DAD1 GO evidence and references
  View Computational GO annotations for DAD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
conditional
null
Resources
86 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • Co-crystal Structure: 1
  • Co-purification: 15
  • Reconstituted Complex: 4
  • Two-hybrid: 26

Genetic Interactions
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 94
Molecular Weight (Da) 10,516
Isoelectric Point (pI) 4.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:478758 to 478474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..285 478758..478474 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002423
References cited on this page View Complete Literature Guide for DAD1
1) Enquist-Newman M, et al.  (2001) Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity. Mol Biol Cell 12(9):2601-13
2) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
3) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
4) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9