RPT6/YGL048C Summary Help

Standard Name RPT6 1
Systematic Name YGL048C
Alias CIM3 2 , CRL3 , SCB68 , SUG1
Feature Type ORF, Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress (3, 4, 5, 6, 7)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Chromosomal Location
ChrVII:411286 to 410069 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -43 cM
Gene Ontology Annotations All RPT6 GO evidence and references
  View Computational GO annotations for RPT6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
345 total interaction(s) for 144 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 129
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 50
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 4
  • Co-localization: 1
  • Co-purification: 10
  • PCA: 5
  • Reconstituted Complex: 13
  • Two-hybrid: 27

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 28
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 3
  • Positive Genetic: 39
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 7
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 405
Molecular Weight (Da) 45,271
Isoelectric Point (pI) 9.71
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:411286 to 410069 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -43 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1218 411286..410069 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003016
References cited on this page View Complete Literature Guide for RPT6
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Ghislain M, et al.  (1993) S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase. Nature 366(6453):358-62
3) Russell SJ, et al.  (1999) Subcellular localization, stoichiometry, and protein levels of 26 S proteasome subunits in yeast. J Biol Chem 274(31):21943-52
4) Xie Y and Varshavsky A  (2000) Physical association of ubiquitin ligases and the 26S proteasome. Proc Natl Acad Sci U S A 97(6):2497-502
5) Glickman MH, et al.  (1999) Functional analysis of the proteasome regulatory particle. Mol Biol Rep 26(1-2):21-8
6) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76