CYS3/YAL012W Summary Help

Standard Name CYS3
Systematic Name YAL012W
Alias CYI1 , FUN35 , STR1 1
Feature Type ORF, Verified
Description Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress (2, 3, 4, 5)
Name Description CYStathionine gamma-lyase
Chromosomal Location
ChrI:130799 to 131983 | ORF Map | GBrowse
Gbrowse
Genetic position: -16 cM
Gene Ontology Annotations All CYS3 GO evidence and references
  View Computational GO annotations for CYS3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 14 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
189 total interaction(s) for 155 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 3
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 3
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 87
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 37
  • Positive Genetic: 21
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 394
Molecular Weight (Da) 42,542
Isoelectric Point (pI) 6.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:130799 to 131983 | ORF Map | GBrowse
SGD ORF map
Genetic position: -16 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1185 130799..131983 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000010
References cited on this page View Complete Literature Guide for CYS3
1) Cherest H and Surdin-Kerjan Y  (1992) Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway. Genetics 130(1):51-8
2) Ono B, et al.  (1991) Regulation of cystathionine gamma-lyase in Saccharomyces cerevisiae. Yeast 7(8):843-8
3) Ono B, et al.  (1992) Cloning and characterization of the CYS3 (CYI1) gene of Saccharomyces cerevisiae. J Bacteriol 174(10):3339-47
4) Cherest H, et al.  (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76