CWH41/YGL027C Summary Help

CWH41 BASIC INFORMATION

Standard Name CWH41
Systematic Name YGL027C
Alias GLS1 , DER7 1
Feature Type ORF, Verified
Description Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description Calcofluor White Hypersensitive 4
GO Annotations All CWH41 GO evidence and references
    View Computational GO annotations for CWH41
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CWH41 Phenotype details and references
Classical genetics
unspecified
Large-scale survey
null
overexpression
Interactions CWH41 All interactions details and references
94 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • PCA: 1
  • Two-hybrid: 1
Genetic Interactions
  • Phenotypic Enhancement: 61
  • Phenotypic Suppression: 5
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 19
  • Synthetic Rescue: 3
Sequence Information
ChrVII:446148 to 443647 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2502 446148..443647 2004-07-20 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002995

CWH41 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CWH41

SUMMARY PARAGRAPH for CWH41

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 5, 6).

CWH41, also known as GLS1, encodes glucosidase I (E.C. 3.2.1.106), an integral membrane protein of the ER (3) which removes the terminal glucose from core oligosaccharides immediately after they are transferred to proteins (7), thereby reversing the reaction catalyzed by Die2p. Although cwh41 mutants are deficient in trimming glucose from core oligosaccharides (2, 8), the downstream effect is usually not severe. Mutants grow, mate, and sporulate normally (3, 8), but have reduced levels of beta-1,6-glucan in the cell wall (3), causing hypersensitivity to the drug Calcofluor White (3, 4) and resistance to yeast K1 killer toxin (3, 4). Proteins with extraneous glucose moieties otherwise mature and are secreted normally (8), although there is a report of failure to degrade certain proteins in the ER (1). Deficiency of the human homolog, GCS1 (OMIM), causes the congenital disorder of glycosylation CDG-IIb (OMIM).

Last updated: 2005-07-12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CWH41]

1) Hitt R and Wolf DH  (2004) DER7, encoding alpha-glucosidase I is essential for degradation of malfolded glycoproteins of the endoplasmic reticulum. FEMS Yeast Res 4(8):815-20
2) Romero PA, et al.  (1997) The yeast CWH41 gene encodes glucosidase I. Glycobiology 7(7):997-1004
3) Jiang B, et al.  (1996) CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly. J Bacteriol 178(4):1162-71
4) Ram AF, et al.  (1994) A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast 10(8):1019-30
5) Herscovics A and Orlean P  (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
6) Burda P and Aebi M  (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
7) Simons JF, et al.  (1998) Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p. EMBO J 17(2):396-405
8) Esmon B, et al.  (1984) Early steps in processing of yeast glycoproteins. J Biol Chem 259(16):10322-7