VPS16/YPL045W Summary Help

Standard Name VPS16 1, 2
Systematic Name YPL045W
Alias SVL6 3 , VAM9 , VPT16 1 , CVT15 4
Feature Type ORF, Verified
Description Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages (5, 6, 7)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXVI:467260 to 469656 | ORF Map | GBrowse
Gene Ontology Annotations All VPS16 GO evidence and references
  View Computational GO annotations for VPS16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
117 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-Western: 36
  • Co-fractionation: 1
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 8
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 798
Molecular Weight (Da) 92,740
Isoelectric Point (pI) 7.04
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:467260 to 469656 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2397 467260..469656 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005966
References cited on this page View Complete Literature Guide for VPS16
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Zheng B, et al.  (1998) Isolation of yeast mutants defective for localization of vacuolar vital dyes. Proc Natl Acad Sci U S A 95(20):11721-6
4) Kim J and Klionsky DJ  (2000) Autophagy, cytoplasm-to-vacuole targeting pathway, and pexophagy in yeast and mammalian cells. Annu Rev Biochem 69:303-42
5) Seals DF, et al.  (2000) A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A 97(17):9402-7
6) Rieder SE and Emr SD  (1997) A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell 8(11):2307-27
7) Peterson MR and Emr SD  (2001) The class C Vps complex functions at multiple stages of the vacuolar transport pathway. Traffic 2(7):476-86