CUP2/YGL166W Summary Help

Standard Name CUP2 1
Systematic Name YGL166W
Alias ACE1 2 , 3
Feature Type ORF, Verified
Description Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication (3, 4, 5, 6)
Chromosomal Location
ChrVII:191129 to 191806 | ORF Map | GBrowse
Gbrowse
Genetic position: -101 cM
Gene Ontology Annotations All CUP2 GO evidence and references
  View Computational GO annotations for CUP2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 31 genes
Regulators 10 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
30 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • FRET: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 10
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 225
Molecular Weight (Da) 24,425
Isoelectric Point (pI) 7.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:191129 to 191806 | ORF Map | GBrowse
SGD ORF map
Genetic position: -101 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..678 191129..191806 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003134
References cited on this page View Complete Literature Guide for CUP2
1) Welch, J.W. and Fogel, S.  (1985) Personal Communication, Mortimer Map Edition 9
2) Thiele DJ  (1988) ACE1 regulates expression of the Saccharomyces cerevisiae metallothionein gene. Mol Cell Biol 8(7):2745-52
3) Buchman C, et al.  (1989) The CUP2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein. Mol Cell Biol 9(9):4091-5
4) Szczypka MS and Thiele DJ  (1989) A cysteine-rich nuclear protein activates yeast metallothionein gene transcription. Mol Cell Biol 9(2):421-9
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Dong K, et al.  (2013) The yeast copper response is regulated by DNA damage. Mol Cell Biol 33(20):4041-50